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Anti inflammatory activity on mice of extract of Ganoderma lucidum grown on rice via modulation of MAPK and nf-kb pathways

ABSTRACT: Ganoderma lucidum is a popular medicinal mushroom with anti-inflammatory potential. In the present study, the aim was to determine the anti-inflammatory effect and mode of action of G. lucidum grown on germinated brown rice (GLBR) in a mouse model of colitis. It was shown that GLBR suppressed the production of nitric oxide (NO) and prostaglandin E2 (PGE2) in lipopolysaccharide (LPS)-stimulated macrophages and decreased the expression of COX-2, TNF-a, iNOS, IL-1b, IL-6, and IL-10 mRNAs. GLBR also inhibited activation of p38, ERK, JNK, MAPKs, and nuclear factor kappa-B (NF-kB). In a mouse model of colitis, colonic mucosal injury was evaluated using macroscopic, biochemical, and histopathological test-ing. Disease activity index (DAI), macroscopic score, and histological score significantly decreased upon GLBR treatment. Moreover, immunofluorescence studies indicated that DSS activates nuclear translocation of NF-kB in colon tissue, which is attenuated by GLBR extract. These findings suggest that GLBR is protective against colitis via inhibition of MAPK phosphorylation and NF-kB activation.


Hasnat, M. A., Pervin, M., Cha, K. M., Kim, S. K., & Lim, B. O. (2014). Anti inflammatory activity on mice of extract of Ganoderma lucidum grown on rice via modulation of MAPK and nf-κb pathways. Phytochemistry. doi:10.1016/j.phytochem.2014.10.019.