ABSTRACT: Two novel polysaccharides termed PLPS-1 and PLPS-2 were isolated from mycelia of cultured Phellinus linteus by hot water extraction, purified by DEAE-52 cellulose and Sephadex G-100 column chromatography, and structurally characterized by FTIR and NMR spectroscopy, GC-MS, periodate oxidation/ Smith degradation, and methylation analysis. The monosaccharide compositions of PLPS-1 (MW 2.5×105 Da) and PLPS-2 (MW 2.8×104 Da) were respectively Glc, Ara, Fuc, Gal, and Xyl in molar ratio 21.964: 1.336: 1.182: 1: 1, and Glc, Gal, Man, Ara, Fuc, Xyl in molar ratio 14.368: 2.594: 1.956: 1.552: 1.466: 1; i.e., both were heteropolysaccharides. The backbone of PLPS-1 consisted primarily of repeating -D-Glc(1 4)- -D-Glc(1 6) units, while that of PLPS-2 consisted of -(13)-D-Glc and -(1 6)-D-Glc. The side branches are also different in their carbohydrate components. In in vitro antitumor assays, PLPS-1 displayed strong anti-proliferative effect against S-180 sarcoma cells through apoptosis, whereas PLPS-2 had no such effect. The difference in antitumor activity between the two PLPS evidently results from their structural differences. PLPS-1 has potential as a novel anticancer agent.
Mei Y, Zhu H, Hu Q, Liu Y, Zhao S, Peng N, and Liang Y. A novel polysaccharide from mycelia of cultured Phellinus linteus displays antitumor activity through apoptosis. Carbohydrate Polymers. Elsevier; 2015.