The effects of freeze-dried Ganoderma lucidum mycelia on a recurrent oral ulceration rat model

Conventional scientific studies had supported the use of polysaccharides and β-glucans from a number of fungi, including Ganoderma lucidum for the treatment of recurrent oral ulceration (ROU). Our aim of the present study was to evaluate whether freeze-dried powder from G. lucidum mycelia (FDPGLM) prevents ROU in rats. Xie, L., Zhong, X., Liu, D., Liu, …

Read More The effects of freeze-dried Ganoderma lucidum mycelia on a recurrent oral ulceration rat model

Two novel diterpenoids, erinacines H and I from the mycelia of Hericium erinaceum

Novel diterpenoids, erinacines H (1) and I (3), were isolated from the cultured mycelia of Hericium erinaceum. The structures of the compounds were determined by interpretation of the spectral data. Erinacine H showed stimulating activity of nerve growth factor (NGF)-synthesis. Lee, E. W., Shizuki, K., Hosokawa, S., Suzuki, M., Suganuma, H., Inakuma, T., . . …

Read More Two novel diterpenoids, erinacines H and I from the mycelia of Hericium erinaceum

Pyrrole alkaloids and ergosterols from Grifola frondosa exert anti-α-glucosidase and anti-proliferative activities

Previously, polysaccharides with hypoglycemic effects from G. frondosa were reported by us. To explore compounds against α-glucosidase and human tumor cells, three extractive fractions (GF-1–GF-3) were prepared from G. frondosa ethanol extraction (GF) by chromatographic column. Rather than GF-1 and GF-2, the fraction GF-3 demonstrated inhibitory actions against α-glucosidase and proliferation of human tumor cells …

Read More Pyrrole alkaloids and ergosterols from Grifola frondosa exert anti-α-glucosidase and anti-proliferative activities

Lanostane triterpenes from the mushroom Ganoderma resinaceum and their inhibitory activities against α-glucosidase

Eighteen previously undescribed lanostane triterpenes and thirty known analogues were obtained from the fruiting bodies of Ganoderma resinaceum. Resinacein C was isolated from a natural source for the first time. The structures of all the above compounds were elucidated by extensive spectroscopic analysis and comparisons of their spectroscopic data with those reported in the literature. …

Read More Lanostane triterpenes from the mushroom Ganoderma resinaceum and their inhibitory activities against α-glucosidase

Ganoderma applanatum secondary metabolites induced apoptosis through different pathways: In vivo and in vitro anticancer studies

Ganoderma applanatum is a widely distributed saprobic or parasitic mushroom, it was found at the bases of decaying logs in Hakozaki Higashi-ku Fukuoka-shi. Japan. The mushroom was extracted with 80% methanol, and LC-HRMS analysis was conducted to illustrate the bioactive ingredients. The cytotoxicity of the total metabolite extract was evaluated against human colon cancer cell …

Read More Ganoderma applanatum secondary metabolites induced apoptosis through different pathways: In vivo and in vitro anticancer studies

Screening of antifungal activity of Ganoderma Lucidum extract against medically important fungi

Antimicrobials of plant origin are effective in the treatment of infectious disease while simultaneously mitigating many side effects that are often associated with synthetic antimicrobials. In the present study crude aqueous and ethanolic extract of Ganoderma lucidum was evaluated for its antifungal activity against the clinically important fungi namely Candida albicans, Aspergillus niger, Penicillium marneffei, …

Read More Screening of antifungal activity of Ganoderma Lucidum extract against medically important fungi

The effects of freeze-dried Ganoderma lucidum mycelia on a recurrent oral ulceration rat model.

Conventional scientific studies had supported the use of polysaccharides and β-glucans from a number of fungi, including Ganoderma lucidum for the treatment of recurrent oral ulceration (ROU). Our aim of the present study was to evaluate whether freeze-dried powder from G. lucidum mycelia (FDPGLM) prevents ROU in rats. Xie, L., Zhong, X., Liu, D., Liu, …

Read More The effects of freeze-dried Ganoderma lucidum mycelia on a recurrent oral ulceration rat model.

Antioxidant activity of liquid cultured Inonotus obliquus polyphenols using tween-20 as a stimulatory agent

Polyphenols are major functional components of the medicinal mushroom Inonotus obliquus . Compared with the wide application of surfactants in medicinal mushroom polysaccharide production, using surfactants to enhance mushroom polyphenol production and antioxidant activity is a relatively new approach. For the first time, in the present study, we evaluated the effect of Tween-20 on the …

Read More Antioxidant activity of liquid cultured Inonotus obliquus polyphenols using tween-20 as a stimulatory agent

GC-MS Profiling of Volatile Components in Different Fermentation Products of Cordyceps Sinensis Mycelia

The fermentation products of Cordyceps sinensis (C. sinensis) mycelia are sustainable substitutes for natural C. sinensis. However, the volatile compositions of the commercial products are still unclear. In this paper, we have developed a simultaneous distillation-extraction (SDE) and gas chromatography-mass spectrometry (GC-MS) method for the profiling of volatile components in five fermentation products. A total …

Read More GC-MS Profiling of Volatile Components in Different Fermentation Products of Cordyceps Sinensis Mycelia

Effects of cordycepin on spontaneous alternation behavior and adenosine receptors expression in hippocampus.

Cordycepin, an adenosine analogue, has been reported to improve cognitive function. Important roles on learning and memory of adenosine and its receptors, such as adenosine A1 and A2A receptors (A1R and A2AR), also have been shown. Therefore, we assume that the improvement of learning and memory induced by cordycepin is likely related to hippocampal adenosine …

Read More Effects of cordycepin on spontaneous alternation behavior and adenosine receptors expression in hippocampus.

Inonotus obliquus extract induces apoptosis in the human colorectal carcinoma’s HCT-116 cell line

Because of irregular dietary habits and lifestyle in Taiwan, the incidence and mortality rate of colorectal cancer have been increasing rapidly these years. This study investigated the inhibitory activity against the proliferation of human colorectal cancer HCT-116 cells by Inonotus obliquus extracts obtained from submerged fermentation. Cell viability was measured by the reduction of MTT …

Read More Inonotus obliquus extract induces apoptosis in the human colorectal carcinoma’s HCT-116 cell line

Culture broth of Ganoderma lucidum exhibited antioxidant, antibacterial and α-amylase inhibitory activities

Culture broth of Ganoderma lucidum was determined for antioxidant, antibacterial and α-amylase inhibitory activities. The culture broth contained protein as determined by Bradford method equaled to 0.2 mg/ml and total phenol content as 0.078 mg GAE/mg protein (0.557 mg GAE/g extract). It exhibited radicals scavenging activities against ABTS+· and DPPH·radicals with a half maximal inhibitory concentration (IC50) less than 1.70 ± 0.02 and 2.28 ± 0.02 µg protein/ml, respectively …

Read More Culture broth of Ganoderma lucidum exhibited antioxidant, antibacterial and α-amylase inhibitory activities

Antimicrobial activity of ethanolic extracts made of mushroom mycelia developed in submerged culture

The ethanolic extracts of dried biomass made of mushroom mycelium produced in the submerged cultures of eight edible/medicinal macromycete species Ganoderma applanatum, Ganoderma lucidum, Laetiporus sulphureus, Flammulina velutipes, Trametes versicolor, Hericium coralloides, Pleurotus eryngii 2600 and Agaricus campestris were evaluated for their antimicrobial activities. Pathogenic tested microorganisms were represented by five bacteria and two yeasts …

Read More Antimicrobial activity of ethanolic extracts made of mushroom mycelia developed in submerged culture

Antitubercular Activity of Mycelium-Associated Ganoderma Lanostanoids.

ABSTRACT: In a continuation of our research into antitubercular lanostane triterpenoids from submerged cultures of Ganoderma species, three strains, Ganoderma orbiforme BCC 22325, Ganoderma sp. BCC 60695, and Ganoderma australe BCC 22314, have been investigated. Fourteen new lanostane triterpenoids, together with 35 known compounds, were isolated. Antitubercular activities of these mycelium-associated Ganoderma lanostanoids against Mycobacterium …

Read More Antitubercular Activity of Mycelium-Associated Ganoderma Lanostanoids.

Polysaccharides from Ganoderma lucidum attenuate microglia-mediated neuroinflammation and modulate microglial phagocytosis and behavioural response.

ABSTRACT: Ganoderma lucidum (GL) has been widely used in Asian countries for hundreds of years to promote health and longevity. The pharmacological functions of which had been classified, including the activation of innate immune responses, suppression of tumour and modulation of cell proliferations. Effective fractions of Ganoderma lucidum polysaccharides (GLP) had already been reported to …

Read More Polysaccharides from Ganoderma lucidum attenuate microglia-mediated neuroinflammation and modulate microglial phagocytosis and behavioural response.

Effects of comb tooth cap medicinal mushroom, Hericium ramosum (higher basidiomycetes) mycelia on DPPH radical scavenging activity and nerve growth factor synthesis.

ABSTRACT: The goal of this study was to evaluate the antioxidant effects of and nerve growth factor (NGF) synthesis caused by Hericium ramosum mycelia. Wild mushroom fruiting bodies were collected from nature to isolate their mycelia. Pieces of H. ramosum fruiting bodies were plated onto 90-mm Petri dishes with potato dextrose agar medium to isolate …

Read More Effects of comb tooth cap medicinal mushroom, Hericium ramosum (higher basidiomycetes) mycelia on DPPH radical scavenging activity and nerve growth factor synthesis.

Phellinus linteus mycelium alleviates myocardial ischemia-reperfusion injury through autophagic regulation.

ABSTRACT: The incidence of myocardial ischemia-reperfusion (IR) injury is rapidly increasing around the world and this disease is a major contributor to global morbidity and mortality. It is known that regulation of programmed cell death including apoptosis and autophagy reduces the impact of myocardial IR injury. In this study, the cardioprotective effects and underlying mechanisms …

Read More Phellinus linteus mycelium alleviates myocardial ischemia-reperfusion injury through autophagic regulation.

Phellinus linteus grown on germinated brown rice increases cetuximab sensitivity of KRAS-mutated colon cancer.

ABSTRACT: Colon cancer is one of the most common types of cancer, and it has recently become a leading cause of death worldwide. Among colon cancers, the v-ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS)-mutated form is notorious for its non-druggable features. Cetuximab, a monoclonal antibody that binds to the epidermal growth factor receptor, has …

Read More Phellinus linteus grown on germinated brown rice increases cetuximab sensitivity of KRAS-mutated colon cancer.

Inonotus obliquus polysaccharide regulates gut microbiota of chronic pancreatitis in mice.

ABSTRACT: Polysaccharide is efficient in attenuation of metabolic ailments and modulation of gut microbiota as prebiotics. The therapeutic effect of Inonotus obliquus polysaccharide (IOP) on chronic pancreatitis (CP) in mice has been validated in our previous study. However, it is not clear whether IOP is conducive to maintaining the homeostasis between gut microbiota and host. …

Read More Inonotus obliquus polysaccharide regulates gut microbiota of chronic pancreatitis in mice.

Effects of cultured Cordyceps mycelia polysaccharide A on tumor neurosis factor-α induced hepatocyte injury with mitochondrial abnormality.

ABSTRACT: Cordyceps sinensis mycelia polysaccharide A (CPS-A), was isolated from cultured Cordyceps mycelia by 65% alcohol extraction and ion-exchange column chromatography. The molecular weight of CPS-A was 1.2 × 104 Da and the backbone was mainly composed of (1 → 2)-linked β-D-mannopyranose, (1 → 2,4)-linked β-D-mannopyranose and (1 → 4)-linked α-D-glucopyranose with terminal β-D-mannopyranose and α-D-glucopyranose residues. CPS-A played a protective role against TNF-α induced mitochondria injury …

Read More Effects of cultured Cordyceps mycelia polysaccharide A on tumor neurosis factor-α induced hepatocyte injury with mitochondrial abnormality.

Preventive effects of Ophiocordyceps sinensis mycelium on the liver fibrosis induced by thioacetamide.

ABSTRACT Thioacetamide (TAA), usually used as a fungicide to control the decay of citrus products, itself is not toxic to the liver, but its intermediates are able to increase oxidative stress in livers and further cause fibrosis. Ophiocordyceps sinensis mycelium (OSM) which contains 10% polysaccharides and 0.25% adenosine decreased (P < 0.05) the lipid accumulation …

Read More Preventive effects of Ophiocordyceps sinensis mycelium on the liver fibrosis induced by thioacetamide.

Identification of a novel component leading to anti-tumor activity besides the major ingredient cordycepin in Cordyceps militaris extract.

ABSTRACT: In accordance with our previous study that was carried out to identify novel anti-tumor ingredients, chromatographic separation in combination with an anti-tumor activity assay was used for analysis of Cordyceps militaris extract in this study. Various modes of chromatography including reversed-phase, cation-exchange and anion-exchange were used to separate components of Cordyceps militaris, which showed …

Read More Identification of a novel component leading to anti-tumor activity besides the major ingredient cordycepin in Cordyceps militaris extract.

The emerging use of mycosterols in food industry along with the current trend of extended use of bioactive phytosterols

ABSTRACT: Background: Consumption of foods enriched with phytosterols (PS) can promote substantial reductions in the low-density lipoprotein cholesterol concentrations, a major risk factor for cardiovascular diseases (CVD). For reaching the 2 g/d threshold dose for cholesterol-lowering purposes, the ingestion of PS- enriched foods as well as PS-based nutraceuticals is required. With a growing global functional …

Read More The emerging use of mycosterols in food industry along with the current trend of extended use of bioactive phytosterols

A mushroom-derived amino acid, ergothioneine, is a potential inhibitor of inflammation-related DNA halogenation.

ABSTRACT: Myeloperoxidase (MPO)-generated halogenating molecules, such as hypochlorous acid and hypobromous acid (HOBr), in inflammatory regions are postulated to contribute to disease progression. In this study, we showed that ergothioneine (EGT), derived from an edible mushroom, inhibited MPO activity as well as the formation of 8-bromo-2′-deoxyguanosine in vitro. The HOBr scavenging effect of EGT is …

Read More A mushroom-derived amino acid, ergothioneine, is a potential inhibitor of inflammation-related DNA halogenation.

Purification and fermentation characteristics of exopolysaccharide from Fomitopsis castaneus Imaz.

ABSTRACT: Short-chain fatty acids (SCFAs), which are the end products of carbohydrate fermentation in the gut, mainly contribute to energy metabolism in mammals. The amount of SCFAs produced during fermentation is an important parameter that characterizes the fermentation capacity of a system. This paper reports on the fermentation characteristics of exopolysaccharides (EPS) isolated from Fomitopsis …

Read More Purification and fermentation characteristics of exopolysaccharide from Fomitopsis castaneus Imaz.

Preventive effects of Ophiocordyceps sinensis mycelium on the liver fibrosis induced by thioacetamide

ABSTRACT: Thioacetamide (TAA), usually used as a fungicide to control the decay of citrus products, itself is not toxic to the liver, but its intermediates are able to increase oxidative stress in livers and further cause fibrosis. Ophiocordyceps sinensis mycelium (OSM) which contains 10% polysaccharides and 0.25% adenosine decreased (P < 0.05) the lipid accumulation …

Read More Preventive effects of Ophiocordyceps sinensis mycelium on the liver fibrosis induced by thioacetamide

Inonotus obliquus polysaccharide regulates gut microbiota of chronic pancreatitis in mice

ABSTRACT: Polysaccharide is efficient in attenuation of metabolic ailments and modulation of gut microbiota as prebiotics. The therapeutic effect of Inonotus obliquus polysaccharide (IOP) on chronic pancreatitis (CP) in mice has been validated in our previous study. However, it is not clear whether IOP is conducive to maintaining the homeostasis between gut microbiota and host. …

Read More Inonotus obliquus polysaccharide regulates gut microbiota of chronic pancreatitis in mice

Enhancement of repair of radiation induced DNA strand breaks in human cells by Ganoderma mushroom polysaccharides

ABSTRACT: The DNA repair ability of a cell is vital to the integrity of its genome and thus to its normal functioning and that of the organism. The repair-enhancing property of polysaccharides isolated from Ganoderma lucidum which belongs to the polyporaceae family was determined by comet assay in human peripheral blood leukocytes. Comet parameters were …

Read More Enhancement of repair of radiation induced DNA strand breaks in human cells by Ganoderma mushroom polysaccharides

Antioxidant, antitumor and immunostimulatory activities of the polypeptide from Pleurotus eryngii mycelium

ABSTRACT: The objective of this study was to investigate the antioxidant, antitumor and immunological activities of the polypeptide from Pleurotus eryngii mycelium (PEMP). The ability of the polypeptide to stimulate Ana-1 macrophages to englobe neutral red, secrete NO, H2O2, TNF-alpha, and IL-6, increase TLR2 and TLR4 expression levels and Ana-1 cell survival rate were investigated …

Read More Antioxidant, antitumor and immunostimulatory activities of the polypeptide from Pleurotus eryngii mycelium