Based on the impact of volatile organic compounds (VOCs) on secondary metabolite pathways, a novel submerged volatile co-culture system was constructed, and the effects of thirteen fungal and bacterial VOCs were investigated on Ganoderma lucidum exopolysaccharides production. The results demonstrated at least a 2.2-fold increase in exopolysaccharide (EPS) specific production yield in 6 days submerged volatile co-culture of G. lucidum with Pleurotus ostreatus. Therefore, P. ostreatus was selected as a variable culture, and the effects of agitation speed, inoculum size, initial pH, and co-culture volume on EPSs production were investigated using a Taguchi L9 orthogonal array. Finally, the highest concentration of EPSs (3.35 ± 0.22 g L1) was obtained under optimized conditions; initial pH 5.0, inoculum size 10%, 150 rpm, and 3:1 volume ratio of variable culture to main culture.
Asadi, F., Barshan-tashnizi, M., Hatamian-Zarmi, A., Davoodi-Dehaghani, F., & Ebrahimi-Hosseinzadeh, B. (2020). Enhancement of exopolysaccharide production from Ganoderma lucidum using a novel submerged volatile co-culture system. Fungal Biology. doi:10.1016/j.funbio.2020.09.010